Pyridinium analogues of green fluorescent protein chromophore: Fluorogenic dyes with large solvent-dependent Stokes shifts

Yu. G. Ermakova, T. Sen, Yu. A. Bogdanova, A. Yu. Smirnov, N. S. Baleeva, A. I. Krylov, and M. S. Baranov
J. Chem. Phys. Lett. 8, 1958 – 1963 (2018)

Novel fluorogenic dyes based on the GFP chromophore are developed. The compounds contain a pyridinium ring instead of phenolate and feature large Stokes shifts and solvent-dependent variations in the fluorescence quantum yield, which facilitates their use for imaging the membrane structure of endoplasmic reticulum (ER). Electronic structure calculations explain the trends in solvatochromic behavior.

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