Pyridinium analogues of green fluorescent protein chromophore: Fluorogenic dyes with large solvent-dependent Stokes shifts

Yu. G. Ermakova, T. Sen, Yu. A. Bogdanova, A. Yu. Smirnov, N. S. Baleeva, A. I. Krylov, and M. S. Baranov
Chem. Comm., submitted (2018)

Novel fluorogenic dyes based on the GFP chromophore are developed. The compounds contain a pyridinium ring instead of phenolate and feature large Stokes shifts and solvent-dependent variations in the fluorescence quantum yield, which facilitates their use for imaging the membrane structure of endoplasmic reticulum (ER). Electronic structure calculations explain the trends in solvatochromic behavior.


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